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notch pathway inhibitor dapt  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc notch pathway inhibitor dapt
    Notch Pathway Inhibitor Dapt, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/notch pathway inhibitor dapt/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    notch pathway inhibitor dapt - by Bioz Stars, 2026-03
    90/100 stars

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    (A, C) Expression of Müller glia marker Glast , retinal pigment epithelium (RPE) marker RPE65 , and Vim in a homeostatic retina and regenerating (28 dpi) contralateral retina; (A) was then probed for Hes1 and (B) for Hes5 . (B) Expression of Hes1 and other Notch pathway genes, receptor Notch1 and ligand Jag1 , in the same retinal tissue section as in (A). Hes1 is expressed in Müller glia in a homeostatic retina. (D) Expression of Hes5 and other Notch pathway genes, Notch1 and Jag1 , in same tissue sections as (C). (E-E’) Proportion of rod photoreceptors in regenerated (101 dpi) retinas after they were treated with either vehicle or <t>Notch</t> <t>inhibitor</t> <t>DAPT</t> at 15 dpi. (F-F’) Proportion of Müller glia in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. Asterisks indicate cells expressing a gene, and arrowheads indicate autofluorescence. Scale bar is 50 μm.
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    (A, C) Expression of Müller glia marker Glast , retinal pigment epithelium (RPE) marker RPE65 , and Vim in a homeostatic retina and regenerating (28 dpi) contralateral retina; (A) was then probed for Hes1 and (B) for Hes5 . (B) Expression of Hes1 and other Notch pathway genes, receptor Notch1 and ligand Jag1 , in the same retinal tissue section as in (A). Hes1 is expressed in Müller glia in a homeostatic retina. (D) Expression of Hes5 and other Notch pathway genes, Notch1 and Jag1 , in same tissue sections as (C). (E-E’) Proportion of rod photoreceptors in regenerated (101 dpi) retinas after they were treated with either vehicle or <t>Notch</t> <t>inhibitor</t> <t>DAPT</t> at 15 dpi. (F-F’) Proportion of Müller glia in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. Asterisks indicate cells expressing a gene, and arrowheads indicate autofluorescence. Scale bar is 50 μm.
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    (A, C) Expression of Müller glia marker Glast , retinal pigment epithelium (RPE) marker RPE65 , and Vim in a homeostatic retina and regenerating (28 dpi) contralateral retina; (A) was then probed for Hes1 and (B) for Hes5 . (B) Expression of Hes1 and other Notch pathway genes, receptor Notch1 and ligand Jag1 , in the same retinal tissue section as in (A). Hes1 is expressed in Müller glia in a homeostatic retina. (D) Expression of Hes5 and other Notch pathway genes, Notch1 and Jag1 , in same tissue sections as (C). (E-E’) Proportion of rod photoreceptors in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. (F-F’) Proportion of Müller glia in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. Asterisks indicate cells expressing a gene, and arrowheads indicate autofluorescence. Scale bar is 50 μm.

    Journal: bioRxiv

    Article Title: Regenerating axolotl retinas regrow diverse cell types with modulation by Notch signaling and reconnect to the brain

    doi: 10.1101/2022.04.28.489898

    Figure Lengend Snippet: (A, C) Expression of Müller glia marker Glast , retinal pigment epithelium (RPE) marker RPE65 , and Vim in a homeostatic retina and regenerating (28 dpi) contralateral retina; (A) was then probed for Hes1 and (B) for Hes5 . (B) Expression of Hes1 and other Notch pathway genes, receptor Notch1 and ligand Jag1 , in the same retinal tissue section as in (A). Hes1 is expressed in Müller glia in a homeostatic retina. (D) Expression of Hes5 and other Notch pathway genes, Notch1 and Jag1 , in same tissue sections as (C). (E-E’) Proportion of rod photoreceptors in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. (F-F’) Proportion of Müller glia in regenerated (101 dpi) retinas after they were treated with either vehicle or Notch inhibitor DAPT at 15 dpi. Asterisks indicate cells expressing a gene, and arrowheads indicate autofluorescence. Scale bar is 50 μm.

    Article Snippet: DAPT treatment: we soaked small pieces (>0.5mm in diameter) of cotton balls (Thermo Fisher) in 100 mM Notch pathway inhibitor DAPT (Tocris) or its vehicle dimethyl sulfoxide (DMSO; Sigma).

    Techniques: Expressing, Marker